unglycosylated

Of note, unglycosylated isoform is underrepresented in all NaPTA precipitated samples as compared with that of the TH preparations (see panel A, lanes 3 and 4 and panel B).

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

The cerebellar unglycosylated PrP res isoform generates a thicker and overall slightly faster migrating band than the corresponding PrP res from the cerebral cortex.

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

C: A high-resolution immunoblot (15%, 15 cm long gel) confirms that the monoglycosylated and unglycosylated PrP res isoforms from the cerebellum have a faster electrophoretic mobility than the corresponding forms from the cerebral cortex, and shows that the cerebellar unglycosylated isoform resolves into three fragments including a 19 kDa band, corresponding to PrP res type 2, and two additional bands of slightly lower relative molecular weight

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

In addition to being PK-sensitive, C1 could be easily distinguished from the unglycosylated form of PrP Immunoblot analysis of total homogenate from brain, pituitary gland and uterus are shown.

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

One exception is the cerebellum where the monoglycosylated and unglycosylated PrP res isoform migrated slightly faster than the PrP res from other brain regions and resolved in three bands.

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

Biosynthesis of an unglycosylated envelope glycoprotein of Rous sarcoma virus in the presence of tunicamycin

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A: Immunoblot of total homogenates (TH), treated with proteinase K (PK), obtained from the frontal cortex of sCJDMM1, sCJDMM2 (representing PrP res types 1 and 2, respectively) and the present case showing the over-representation of the upper band (Diglyc.) containing the diglycosylated form, and the co-migration of the lowest band (Unglyc.), containing the unglycosylated form, with the corresponding band of sCJDMM2.

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

When a high resolution gel (15%, 15 cm long) was used, the PrP res unglycosylated form in the cerebellum appeared to resolve into three bands, which included the band corresponding to the PrP res type 2 of 19 kDa and two additional bands that migrated about 0.5 kDa and 1 kDa faster (

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010

Generally, in the NaPTA preparations the unglycosylated form was less well represented than in the TH preparations (

PLoS ONE Alerts: New Articles Silvio Notari et al. 2010