vibratome

Results are presented in Coronal slices (100-µm thick) of rat brains were cut with a VT 1000S vibratome (Leica) and directly fixed for 1 h in iced 4% paraformaldehyde in phosphate-buffered solution (PBS).

PLoS Biology: New Articles Marta Gómez-Gonzalo et al. 2010

Coronal slices were obtained by cutting with a Leica vibratome VT1000S in the presence of the ionotropic glutamate receptor inhibitor kynurenic acid (2 mM).

PLoS Biology: New Articles Marta Gómez-Gonzalo et al. 2010

Immunohistochemistry on vibratome slices of paraformaldehyde-fixed tissue was performed using standard procedures as described Functional complementation of NCre and CCre in vitro.

PLoS ONE Alerts: New Articles 2009

For cross sections, fins were embedded in standard condition and section (20 µm) performed using a vibratome.

PLoS ONE Alerts: New Articles Mohamed Bouzaffour et al. 2009

Retinas from 6-8 week old C57BL / 6J mice were dissected and flattened by making 4 radial cuts, then placed on a 20\% gelatin block secured to a vibratome chuck.

PLoS ONE Alerts: New Articles Magali Saint-Geniez et al. 2008

Photoreceptor cells collected by enzymatic digestion (papain) of vibratome-isolated photoreceptor sheets were cultured in 96 well laminin-coated plates (Sigma) at 37°C under 5\%

PLoS ONE Alerts: New Articles Magali Saint-Geniez et al. 2008

Fifty-micrometer horizontal vibratome sections were then cut, cryoprotected in 2\% glycerol and 20\% DMSO in 0.1 M PB for 20 min, and freeze-thawed eight times in liquid nitrogen.

PLoS Biology: New Articles 2008