Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biochemistry A form of
electrophoresis used in the study ofenzyme activity
Etymologies
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Examples
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Secretion of MMP-2 was evaluated by gelatin zymography, as previously described (Barille et al. 1999).
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HA, s-GAG and MMP-2 releases to media were measured using ELISA, dye-binding assay and gelatin zymography, respectively.
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Hypoxia increased gelatinolytic activity specifically in vascular structures and gel zymography identified MMP-9 as enzymatic source.
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The expression and activity of matrix metalloproteinase 2 (MMP-2) within the tumor were determined by immunohistochemitry and zymography.
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Western blot shows tPA protein level (a) and zymography shows tPA activity (b) in MCAo mice with or without MSC treatment. tPA and PAI-1 expression was significantly increased in the IBZ of mice subjected to MCAo compared with normal mice. tPA expression was significantly increased and PAI-1 expression was significantly decreased in the IBZ of MCAo mice after MSC treatment compared with MCAo alone mice (c).
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In this regard, this activated form could be detected by zymography in supernatants of all melanoma cell lines that cross-presented the
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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS / PAGE) zymography was performed for determination of gelatinase activity as described before
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The proteolytic activity of MMP-9 measured by zymography was also significantly diminished in mice treated daily with i.p. injections of minocycline and i.v. injections of PEG minocycline-liposomes every five days (B).
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Proteolytic activity of MMP-9 was assessed in supernatants of human PBMCs after 24 hrs of stimulation with IL-2 and assessed by gelatin-zymography, as previously described
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Gelatinolytic activity was detectable by gelatin-zymography at molecular weights of 92 kDa, indicative of MMP-9, in the supernatants from all human PBMC samples studied.
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