from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. A hydroxy derivative of the amino acid proline found in the structural proteins of connective tissue
from the GNU version of the Collaborative International Dictionary of English
- n. A crystalline amino acid (C5H9NO3), a hydroxylated proline, obtained by hydrolysis of gelatin or collagen. Chemically it is 4-hydroxy-L-proline. It is classified as nonessential for growth in rats.
from WordNet 3.0 Copyright 2006 by Princeton University. All rights reserved.
- n. a crystalline amino acid obtained from gelatin or collagen
Sorry, no etymologies found.
On the presence of collagen in the brain of calf and man with special reference to the isolation of an unknown protein containing hydroxyproline by Leonard Wartofsky
These animal express collagen, but it doesn't contain hydroxyproline.
As a clarification of the above, molecular oxygen, as I understand it, is necessary for the post-transcriptional hydroxylation of proline to hydroxyproline - ie the oxygen in the hydroxyproline does not come from water, but is added from an O2 molecule by an oxygenase enzyme.
• X-ray diffraction studies reveal a cylinder of water surrounding the triple-helix which is hydrogen-bonded to the hydroxyproline side-chains.
Almost one third of the amino acids are glycine and one quarter is proline or hydroxyproline.
Most of the polypeptide chain is a repeating unit of the tripeptide glycine-X-proline, where X is another amino acid and proline can be hydroxyproline.
Hydroxyproline was quantified by reverse-phase high performance liquid chromatography (HPLC) of 7-chloro-4-nitrobenzo-oxao-1,3, - diazole - derived acid hydrolysates; the total left lung collagen was then calculated in milligrams, assuming that lung collagen contains 12. 2% (w/w) hydroxyproline.
The total collagen content of the left lung was determined by measuring hydroxyproline content in aliquots of pulverized left lung as described previously
Interestingly KGF levels correlated with hydroxyproline content of the lungs after bleomycin injury in controls but not in HSC-KGF treated mice (which we would have predicted to be lower due to the reduced injury), suggesting that the raised levels were indeed due to the local KGF delivery by bone marrow derived cells
In contrast, HPLC determination of hydroxyproline content, as a surrogate marker for collagen protein levels, demonstrated no significant differences between the bleomycin groups - in agreement with the Ashcroft scores.