Definitions

from The American Heritage® Dictionary of the English Language, 5th Edition.

  • noun A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies.

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun A blot used in western blot analysis
  • verb To perform such an analysis

Etymologies

from Wiktionary, Creative Commons Attribution/Share-Alike License

immuno- +‎ blot

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Examples

  • GM immunoblot showed an IgE-binding 14 kDa band that was totally inhibited after serum pre-incubation with GM.

    Archive 2007-08-01 Steve Carper 2007

  • GM immunoblot showed an IgE-binding 14 kDa band that was totally inhibited after serum pre-incubation with GM.

    Goat's Milk Allergy Steve Carper 2007

  • Screen with FAST-ELISA and confirm with immunoblot.

    Schistosomiasis 2008

  • A recombinant immunoblot assay (RIBA) that identifies antibodies which react with individual HCV antigens is often used as a supplemental test for confirmation of a positive EIA result.

    Chapter 2 2000

  • Lanes 1-5, immunoblot analysis of cell extracts before immunoprecipitation (input).

    Naturejobs - All Jobs Daniela Di Bella 2010

  • Huh7 cells were transfected with pCI-vTK and G418-resistant Huh cellular clones (cl) (1-6) were evaluated for vTK expression (~43 KDa, arrowheads) by immunoblot analysis.

    PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

  • Figure 6: Magoh acts upstream of the microcephaly-associated protein Lis1 to regulate neurogenesis. gene expression measured by quantitative PCR (left) and Lis1 protein expression measured from immunoblot analyses (right).

    Naturejobs - All Jobs Debra L Silver 2010

  • Cellular expression of vTK protein was detected by immunoblot analysis essentially as described

    PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010

  • Substitutions affecting respiration are in bold. (a) Fluorescence immunoblot analysis with anti-MrpL32 shows that yeast MrpL32 precursor (p) accumulates in yta10Δyta12Δ m-AAA-deficient cells (lane 2). m, mature MrpL32.

    Naturejobs - All Jobs Daniela Di Bella 2010

  • P > 0.05. (b) Fluorescence immunoblot analysis and protein quantitation of mitochondria-encoded COX subunits Cox1p, Cox2p and Cox3p, and nuclear-encoded subunit Cox4p, in mitochondrial extracts from cells expressing AFG3L2 alone, in the absence of paraplegin.

    Naturejobs - All Jobs Daniela Di Bella 2010

Comments

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  • with thanks to sionnach for the suggestion.

    October 28, 2007