immunoprecipitation love

immunoprecipitation

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A technique in which an antigen is precipitated from solution by using an antibody, or a particular use of this technique

Etymologies

immunoprecipitate +‎ -ion (Wiktionary)

Examples

  • DNA sequencer and chromatin immunoprecipitation techniques is desirable.

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  • Chromatin immunoprecipitation (ChIP) assay confirmed the recruitment of transcription factors phospho ATF-2, Sp1, Sp3, PPARγ, chromatin modifier histone deacetylase 1 (HDAC1) and the acetylated form of histone H3 but not cRel, C/EBP-α and phospho c-Jun during resistin gene transcription.

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  • Additionally, a physical interaction between PPARγ and Sp1 or Sp3 was evident from co-immunoprecipitation experiments.

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  • Indeed co-immunoprecipitation assay demonstrated a direct physical interaction of Sp1 with PPARγ in whole cell extracts of U937 cells.

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  • However, analysis of the known sequences of eukaryotic miRNAs and co-immunoprecipitation experiments indicate that there is a clear bias for U or A at the 5′ position

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  • This panel also includes co-immunoprecipitation between overexpressed BIN1-V5 (IP) and Cav1. 2 (IB) in HeLa cells.

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  • Co-immunoprecipitation of BIN1 and Cav1. 2 (Figure 4B) further indicates that they are present in the same protein complex.

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  • Studies involve freshly acquired human and mouse adult cardiomyocytes using complementary immunocytochemistry, electron microscopy with dual immunogold labeling, and co-immunoprecipitation.

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  • Methods: The ribonomic approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs.

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  • AKAP5 also associates with PSD-95 and GluR2/3 by immunoprecipitation but the interaction between GluR2/3 and PSD-95 is not disrupted in the AKAP5 KO.

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