Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun biology All the material formed by the lysis of cells

Etymologies

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Examples

  • The lysate is then filtered and placed on the ion-exchange column.

    Archive 2005-10-01

  • ( "lysate") and GST-Plk1-PBD associated 53BP1 were analyzed by immunoblotting using anti-GFP antibody.

    PLoS Biology: New Articles

  • Most striking was the finding that depletion of haem, and addition of GSSG or dsRNA all seemed to have similar effects on cell-free protein synthesis in the reticulocyte lysate, as though these disparate agents all acted in a similar manner.

    Tim Hunt - Autobiography

  • One other thing I learned about during the studies on the inhibition of protein synthesis by dsRNA was that micrococcal nuclease, which requires Ca2+ for activity, did not inhibit protein synthesis in the reticulocyte lysate provided no Ca2+ was present.

    Tim Hunt - Autobiography

  • I also discovered that simple gel-filtration of the lysate caused a drastic fall in its activity.

    Tim Hunt - Autobiography

  • By 1977, the outlines of all this were pretty clear, and we had a very good understanding of the reticulocyte lysate and its properties.

    Tim Hunt - Autobiography

  • The micrococcal nuclease calcium dependence was put to good use by Hugh Pelham, a graduate student in the lab, who developed the use of nuclease treatment of the lysate to assay heterologous mRNAs.

    Tim Hunt - Autobiography

  • In the left panel, 3% of the lysate input used in the immunoprecipitations was subjected to western blot analysis using an anti-ubiquitin antiserum to show that general ubiquitination levels were similar in all genotypes.

    PLoS ONE Alerts: New Articles

  • Plasma membrane and total cell lysate proteins were resolved by SDS-PAGE, blotted on PVDF membrane and probed with anti-GLUT4 antibody.

    PLoS ONE Alerts: New Articles

  • In the left panel, 3% of the lysate input used in the immunoprecipitations was subjected to western blot analysis using an anti-ubiquitin antiserum to show that general ubiquitination levels were similar in all genotypes.

    PLoS ONE Alerts: New Articles

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