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We have deer, gopher tortoises, piliated woodpeckers, armadillos, and many other wild creatures, some of whom are becoming unconscionably rare elsewhere.
In terms of biofilm formation these mutants, which tend to release polymerized pili into the culture supernatants, behave like a non piliated strain.
To support this finding, we generated an isogenic knock-out (KO) mutant strain 515ΔPI-2a in which the whole pilus island 2a was deleted and we complemented this non piliated strain with each of the three pilus islands identified in GBS.
We feel like it's summer here too because it's in the 40s! just took a nice walk to the other end of the lake where we saw a giant piliated woodpecker! they're Huge and make the classic woody woodpecker call. made my day.
I've seen a tree full of ibis at sunset, red-shouldered hawks flying overhead in the morning, piliated woodpeckers drumming away, splashy river otters (and their less-welcome imported cousins nutria), a host of turtles and snakes, alligators (a bit of a drive to get to Alligator River, but zomg!), and lots of very, very cool-looking trees and plants.
This observation could be of relevance for other piliated Gram-positive pathogens, including GBS isolates analyzed in this work were collected by the Center for Disease Control and Prevention (CDC), Atlanta (100 strains); Baylor College of Medicine (BCM), Houston (103 strains) and Istituto Superiore di Sanità, Italy (86 strains).