from The American Heritage® Dictionary of the English Language, 4th Edition
- n. An enzyme that catalyzes the hydrolysis of DNA.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. deoxyribonuclease
from the GNU version of the Collaborative International Dictionary of English
- n. any of numerous enzymes that catalyze the breakdown of DNA into oligonucleotides or mononucleotides.
To test this hypothesis, I added DNase to the crude extract in which we monitored ATP - and ubiquitin-dependent degradation of BSA, that was used as one of our model substrates.
The contiguous primers annealing to the common exon of the B19 genome were used for both DNA and RNA detection, the latter after DNase treatment.
Upon completion, the reaction mixtures were treated with DNase RQ1 (Promega) at
Manufactured in clean room conditions BRAND PCR products are DNase, DNA - and
They have discovered that the novel effect of caspase 3 in stem cells is related to its ability to activate another protein that cuts up the cell's DNA (called caspase-activated DNase) and has also traditionally been associated with programmed cell death.
On-column DNase I digestion was performed. 100 ng of each RNA sample was retrotranscribed with QuantiTect Whole Transcriptome Kit (Qiagen) and a 50-fold dilution of cDNAs was analyzed by quantitative Real Time PCR (qRT-PCR) using an iCycler-iQ5
CAD (or caspase-activated DNase in full) by slicing apart other proteins holding it at bay.
DNA was removed in two steps, on-column using Qiagen RNase-free DNase and after elution using RNase-free DNase (New England Biolabs).
RNA was protected with 5 mM EDTA prior to DNase inactivation. cDNA was generated with the Takara RNA PCR Kit (AMV) with 500 ng of RNA as template and random 9-mers.
DNase and a protease inhibitor cocktail (Sigma-Aldrich) were added.
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