Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. a cross-linked polysaccharide bead-formed gel based on agarose.

Etymologies

Sorry, no etymologies found.

Examples

  • Human seminal plasma from subjects with normal semen parameters were separated on a gelatin-Sepharose column and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting using antibodies against distinct FN forms.

    Naturejobs - All Jobs

  • The lysate was then incubated with either mouse anti-V5 antibody (2 µg) or equal amount of non-specific mouse IgG for 2 h before pulldown with rec-protein-G-Sepharose

    PLoS Biology: New Articles

  • Protein G Sepharose (GE Healthcare, Piscataway, NJ) beads were then added to the lysate for 3 hours, collected by low-speed centrifugation, washed three times in lysis buffer and boiled in 2X SDS-PAGE sample buffer with 2% beta-mercaptoethanol for 10 minutes to release immunoprecipitated dMfn.

    PLoS ONE Alerts: New Articles

  • Clarified cell lysate was loaded onto a Nickel Chelating Sepharose Fast Flow column (GE healthcare), the His-tagged SSADH protein was eluted in 150 mM NaCl, 250 mM imidazole, 5 mM β-mercaptoethanol, 50 mM Tris (pH 8.0).

    PLoS ONE Alerts: New Articles

  • His6-pirin was incubated with glutathione-Sepharose beads that were conjugated to GST or GST-Bcl3 ARD.

    Naturejobs - All Jobs

  • Protein G-Sepharose beads were blocked in 0.1% BSA in binding buffer for 2 hours.

    PLoS ONE Alerts: New Articles

  • After purification with an Ni Sepharose ™ 6 Fast Flow (GE Healthcare), rSeTre-1 and rSeTre-2 were used to immunize rabbits as described previously

    PLoS ONE Alerts: New Articles

  • Recombinant proteins were purified by affinity chromatography using Glutathione Sepharose 4B (Amersham).

    PLoS ONE Alerts: New Articles

  • Intact GST-fusion proteins were incubated with glutathione Sepharose 4B beads (GE Healthcare) for 2 h.

    PLoS Biology: New Articles

  • Immuno-complexes were precipitated by the addition of ~20 µl IgG-Sepharose beads and incubation for 2 hours, followed by spinning the beads down (1000×g, 30 s) and washing 5 times with ice-cold IP buffer.

    PLoS ONE Alerts: New Articles

Wordnik is becoming a not-for-profit! Read our announcement here.

Comments

Log in or sign up to get involved in the conversation. It's quick and easy.