“Repeated attempts had been made to cure the affected areas with topical antimycotic drugs, antibiotics, and virostatic agents.”
“The sorted MiaPaCa2 cells were suspended in serum-free culture medium DMEM containing 1% N2 supplement, 2% B27 supplement, 1% antibotic-antimycotic (Invitrogen), 20 ng/ml human FGF-2”
“The biopsy was immediately minced using sterilized forceps in a 60 mm dish containing 10% DMEM F12 media with 2.5 mg/l antibiotic/antimycotic (Invitrogen Life Sciences, Carlsbad, CA) and incubated for 10-14 days until fibroblasts started to attach to the plate.”
“Of the ten antimycotic compounds within the chemical library, all but three (flutrimazole, thiabendazole, and griseofulvin) were identified in the preliminary screen.”
“A suspension of primary epidermal cells was prepared in keratinocyte serum-free medium (Keratinocyte SFM, Invitrogen; supplemented with antibiotic / antimycotic solution, Sigma-Aldrich) at a density of 4×10”
“(Hyclone), 2% L-glutamine (Gibco®), 20 U/ml γIFN (Chemicon), 1% chick embryo extract (US Biological) and 1X antibiotic-antimycotic (Gibco®).”
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