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This is especially true for enhanced cyan fluorescent protein, which can be excited off-peak by an argon-ion laser (using the 457-nanometer spectral line) and is significantly more resistant to photobleaching than the blue derivative.
Nikon system mounted on Nikon TE2000 microscope and argon-ion laser with 514 nm line and diode laser with 440 nm line, dichroic beam splitter 505 nm, emission filters 470 / 30 nm and 550 / 30 nm.
Developers of argon-ion lasers and their rivals take note, researchers in France have unveiled a new challenger.
In the past few years, numerous research groups and companies have sought to replace the argon-ion laser with a compact and efficient alternative.
"The goal is to replace the argon-ion in a large number of applications, and in particular in biology."
Illustrated in Figure 6 (a) is a transfected living mammalian cell containing PA-GFP in the cytoplasm being imaged with 488-nanometer argon-ion laser excitation before (Figure 6 (a)) and after (Figure 6 (d)) photoconversion with a 405-nanometer blue diode laser.
Zecotek's visible wavelength fiber lasers have been specifically developed as higher performance, lower cost replacements for argon-ion and solid-state laser wavelengths in applications including: bio-instrumentation (DNA Sequencing, Flow Cytometry,
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