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Merged bright-field and aniline-blue stained UV fluorescence images showing the correspondence between xylem and phloem-associated callose
To assess motility in a sample, at least 200 sperm in two independent aliquots were classified as having progressive or non-progressive motility, or being immotile (bright-field microscopy, 40×).
It has a specially designed microscope that operates with bright-field, phase-contrast and fluorescence modes.
Nissl-stained sections were examined under a microscope under bright-field illumination.
Arrows indicate the locations of nucleus HVC in each as identified under bright-field illumination in an adjacent Nissl stained section, arrowheads indicate the location of area X. Scale bar: 1 mm.
(D) High magnification bright-field views of emulsion autoradiography depicting examples of high expression (black grains) over the majority of large HVC cells (C; Arrows), a portion of these cells (D), or virtually all large and small cells (E) in sections hybridized with antisense probes directed against follistatin
HVC was identified under bright-field illumination by the characteristic bump on the brain surface and the presence of clusters of large - to medium-sized ovoid-shaped cells oriented parallel to the overlying ventricle (
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