co-transfected

The HeLa cells were co-transfected with sub-cellular localization vectors fused to enhanced cyan and yellow (EYFP) fluorescent protein coding sequences (Golgi complex and the nucleus, respectively), as well as a variant of the Discosoma striata marine anemone fluorescent protein, DsRed2FP, targeting the mitochondrial network.

Archive 2005-10-01 2005

To access Cav1. 2 and BIN1 colocalization by TIRFm, HeLa cells were plated overnight and co-transfected with pDest-BIN1-mCherry, and Cav1. 2-GFP along with β2b and a2δ1.

PLoS Biology: New Articles Ting-Ting Hong et al. 2010

In parallel experiment, HEK cells were co-transfected with plasmid constructs carrying resistin gene promoter, with or without Sp1 cognate motif, along with Sp1 over-expression plasmid and the expression of luciferase reporter gene was monitored.

PLoS ONE Alerts: New Articles Anil K. Singh et al. 2010

The cells were co-transfected with growth hormone-1

Naturejobs - All Jobs Alfons Meindl 2010

Quantitative analysis of 53R mRNA levels in cells co-transfected with different plasmids.

PLoS ONE Alerts: New Articles Yu-Sin Kim et al. 2010

For live-cell imaging of microtubule behavior by spinning disc confocal microscopy, Hela cells were plated and co-transfected with pDest-BIN1-mCherry and α-tubulin-GFP.

PLoS Biology: New Articles Ting-Ting Hong et al. 2010

To investigate complex formation, RGC5 cells were co-transfected with pTarget-FLAG-OPTN and pRab8

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

In all experiments the Wnt-responsive reporter plasmid TOP.lash (Upstate, #21-170) was co-transfected with a CMV-driven ß-Galactosidase reporter plasmid (kindly provided by Dr.P. Ducy, New York, USA) to normalize for transfection efficiency.

PLoS ONE Alerts: New Articles Jochen Schulze et al. 2010

RGC5 and RPE cells were transfected either with pTarget-FLAG-OPTN alone or co-transfected with pRab8

PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010

Luciferase reporter gene, only when Wnt1 or Wnt3 expression plasmids are co-transfected, but that

PLoS ONE Alerts: New Articles Jochen Schulze et al. 2010