from The American Heritage® Dictionary of the English Language, 4th Edition
- n. Genetics A hybrid vector that has been spliced with plasmid DNA for cloning large genes or gene fragments.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. A type of plasmid (often used as a cloning vector) constructed by the insertion of cos sequences, which are DNA sequences of the lambda phage.
from WordNet 3.0 Copyright 2006 by Princeton University. All rights reserved.
- n. (genetics) a large vector that is made from a bacteriophage and used to clone genes or gene fragments
E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair
The alphaT2ib open reading frame was integrated into an adenoviral cosmid vector for production of recombinant adenovirus (AdV) - alphaT2ib.
However, the linearized cosmid produced an incomplete assembly because the two DNA termini, though greatly over-represented in the clone library used for sequencing, were separated from neighboring sequences by gaps of ~1. 4 and 1.8 kb.
These gap sizes were reduced, but not eliminated, by shearing the linear cosmid into smaller fragments.
1987 (Burke et al. 1987) permitted much larger segments of DNA to be ordered and stored for sequencing than was possible with plasmid or cosmid libraries.
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