dithiothreitol love

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A small-molecule redox reagent with the molecular formula C4H10O2S2.

Etymologies

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Examples

  • Samples were subjected to reduction with dithiothreitol, alkylation with iodoacetamide, and in-gel digestion with trypsin or chymotrypsin overnight at pH 8.3, followed by reversed-phase microcapillary/tandem mass spectrometry (LC/MS/MS).

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  • Here we show that addition of dithiothreitol to the low-speed pellet disrupted the polymeric network, presumably by reduction of disulfide bonds linking the monomers.

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  • Also, by shifting the Tamm-Horsfall protein to the high-speed pellet, the use of dithiothreitol makes it feasible to use Tamm-Horsfall protein to normalize excretion rates of exosomal proteins in spot urines.

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  • CBB, Coomassie brilliant blue R-250; DTT, dithiothreitol; 1D-PAGE, one-dimensional polyacrylamide gel electrophoresis; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; ECM, extracellular matrix; GlcNAc,

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  • Alternatively, immunoprecipitations were subjected to in vitro phosphorylation by resuspension in kinase buffer (50 mM Tris-HCL pH7. 5, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT, 2 mM dithiothreitol, 0. 01% BRIJ35, and 150 mM NaCl2), followed by addition of

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  • Figure 4A also shows that the reducing reagent dithiothreitol

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  • Cells were washed once with PBS and resuspended in ice-cold sucrose buffer (250 mM sucrose, 0.5 mM EGTA, 20 mM Hepes-K, pH 7.0) containing 2 mM dithiothreitol.

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  • -80 C, microsomal preparations containing 10% sucrose, frozen in li - quid nitrogen, stable [3], -70 C, sucrose or glycerol plus dithiothreitol stabilize during purifica - tion and storage [5], -70 C, storage of microsomes in presence of 14 mM mercaptoethanol and 15% sucrose, stable for several weeks [7] 335

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  • NADPH, dithiothreitol, 2-mercaptoethanol and as - corbic acid are added alone to the reaction mixture, stimulation when added together with tetrahydropteridine [2]; required as prosthetic group, 291

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  • -20 C, 0.1 M phosphate buffer, pH 7, 0.1 mM dithiothreitol, 1 mM EDTA [6, 53] References [1]

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