- n. biotechnology the creation of transient pores in the cell membrane, through the application of a high-voltage electrical pulse
- electro- + poration (Wiktionary)
“The authors used a nifty technique called electroporation to label the cells in that region so they could watch them as the embryo grew.”
“His initial work was based upon the research of Dr. Kaali and Dr. Lyman who discovered is 1990 that small electrical currents applied to blood would kill microbes in the blood stream as well as increase the porosity of cellular membranes (referred to as electroporation).”
“HIV vaccine testing a novel delivery mechanism known as electroporation - the use of electrical pulses to increase the immune responses elicited by DNA vaccines - was recently completed in New York City.”
“One of the most widely used physical methods to deliver genes into cells "is incredibly inefficient because only a small fraction of a cell's total membrane surface can be permeated," said Lu, an associate professor of chemical engineering at Virginia Tech. The method Lu is referring to is called electroporation, a phenomenon known for decades that increases the permeability of a cell by applying an electric field to generate tiny pores in the membrane of cells.”
“How To Make a Neuron Grow title Single Cell Electroporation in vivo within the Intact Developing Brain description How to perform single cell electroporation, using a micropipette to introduce DNA into a single neuron in tadpole brains.”
“Specialized equipment is necessary for electroporation, however, the technique is comparable in expense to lipofection reagents when a large number of transfections are performed.”
“In this regard, electroporation has proven to generate stable transfectants with linearized plasmids and purified genes.”
“The above mentioned gene transfer systems have become less popular since the advent of electroporation, a technique involving the application of high-voltage electric pulses of short duration to induce the formation of transient pores in cell walls and membranes.”
“Since the recombinant DNA is a naked DNA molecule, gene transfer systems based on protoplast transformation and electroporation are most applicable in genetic engineering experiments.”
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