eluted

• Proteins bound to the columns were eluted with glycine (pH 2.7), digested with a set of proteases (varying combinations of trypsin, subtilisin, elastase, and endopeptidase Lys-C) to achieve higher sequence coverage, and analyzed by MS. —  PLoS Biology: New Articles
• Peptide mixtures were pressure-loaded onto a RP or strong cation exchange / RP MudPIT column and eluted with a linear gradient of acetonitrile or pulses of ammonium acetate salt (5\%-100\%). —  PLoS Biology: New Articles
• The resulting extracts were bound to the antibody columns, and arginylated proteins were eluted with Gly (pH 2.7) or 3.5 M MgCl —  PLoS Biology: New Articles
• The beads were subjected to four rounds of washing with 1 ml of lysis buffer, then immunoprecipitates were eluted by adding electrophoresis sample buffer. —  PLoS ONE Alerts: New Articles
• Figure S1A), where activity eluted at about 300 mM NaCl. —  PLoS ONE Alerts: New Articles

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