“For many years, the method of choice for supercoiled plasmid and virus DNA purification was cesium chloride density gradient centrifugation in the presence of an intercalation agent (such as ethidium bromide or propidium iodide).”
“If the occasion is pouring an ethidium bromide gel, then a "pants-suit" (blech) is not practical.”
“Today I had to find the ethidium bromide, nevermind where to dispose of anything that had touched it.”
“There was no sign by ethidium bromide of any 400-bp bands.”
“Generation of amplicons of the expected molecular sizes was verified by gel electrophoresis and ethidium bromide staining as per usual methods”
“PCR products were visualised following electrophoresis on a 1. 5% agarose gel stained with ethidium bromide (0.5 µg/ml).”
“A final amplification product of about 347 bp was then analyzed by electrophoresis through a 1. 0% agarose gel and visualized by ethidium bromide staining.”
“Digestion products were visualized by ethidium bromide staining following electrophoresis on a 4% agarose gel.”
“For membrane integrity, ethidium homodimer-1 (EthD-1; 4.5 µM final concentration) was added at the indicated time points and allowed to incubate for 30 min at room temp.”
“Intercalation of ethidium bromide with the isomeric deoxytetranucleoside triphosphates 5-d (GpCpGpC) and 5-d (CpGpCpG) in aqueous solution.”
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