Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A potent antifungal chemical first isolated from the actinomycete Streptomyces filipinensis, widely used as a histochemical stain for cholesterol.

Etymologies

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Examples

  • TNM-BF (upper right panels and red in merged images) and filipin (lower left panels and green in merged images) signals were observed in the similar region.

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  • The upper panels show a differential interference contrast micrograph of a yeast cell (left) and a fluorescent image of the cell stained with the membrane probe filipin

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  • The lower panels show a fluorescent image of a cell stained with TNM (left) and a merged image (right) of the cell labeled with TNM (red) and stained with filipin (green).

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  • By synthesizing fluorescently labeled TNM derivatives and comparing their in vivo localization to filipin molecules-known membrane-binding compounds-the team discovered that

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  • No differences were observed in either filipin or GM1 labeling between CD-treated and untreated GM1 mice.

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  • Analysis of cholesterol accumulation by filipin labeling revealed a decrease in cholesterol storage in neocortical neurons of combination and CD-treated

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  • Images of filipin labeling were obtained on the same Olympus microscope but utilizing settings appropriate for acquisition of fluorescent images.

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  • Overall, filipin labeling of lung from both untreated and CD-treated

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  • To visualize unesterified cholesterol, sections were incubated with filipin complex (0. 005% dissolved in DMSO and diluted in PBS) or DMSO

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  • However, most Purkinje cells in age-matched CD-treated Npc1 −/− mice lacked cholesterol storage and only a small number of filipin-positive neurons were present in the granule cell layer.

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