galactopyranoside

E. coli MC4100 or V. parahaemolyticus strains, each harboring a reporter plasmid were grown for 1 h at 37°C in LB broth containing 1.0 or 0. 5% NaCl. β-galactosidase activity was assayed in cell lysates by Miller's method using o-nitrophenyl-β-D-galactopyranoside (ONPG) as a substrate The promoter region of vtrB, containing a 284 bp upstream sequence of the start codon, was amplified by polymerase chain reaction (PCR).

PLoS ONE Alerts: New Articles Toshio Kodama et al. 2010

E. coli BL21 (λDE3) cells were transformed with pET28c. fad, the transformants were grown to mid-logarithmic phase in LB media containing 25 µg/ml of kanamycin and synthesis of FadD13 protein was induced by the addition of 1 mM isoproryl-1-thio-β-D-galactopyranoside (IPTG) and the cells were harvested after incubation at 30°C for 3 hours with a constant shaking at 200 rpm.

PLoS ONE Alerts: New Articles Garima Khare et al. 2009

[36] was used as a transfection control in various experiments as indicated. β-galactosidase activity was measured by the chlorophenol red-β-D-galactopyranoside (CPRG) - based assay HeLa cells were grown on coverslips and transfected with plasmids as above.

PLoS ONE Alerts: New Articles Luke W. Meredith et al. 2009