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“On the ends of the chains, Mynar created multiple branches tipped with guanidinium, a chemical relative of guanine, a molecular building block of DNA and RNA.”
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“In reduced mutant Spx. the side chain of residue R92 has rotated outward by ~2. 8 Å from its location in oxidized Spx. In the oxidized protein, the guanidinium side chain is engaged in an electrostatic interaction with a bound sulfate ion”
“If so, this also places the solvent exposed guanidinium group of residue R60 near the DNA phosphate backbone and suggests its possible role in DNA binding, either to the backbone or to a guanine.”
“Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.”
“The membranes were dried for 1 h at 37°C, incubated with 0.5 µg / mL PK in lysis buffer for 90 min at 37°C followed by treatment with 3 M guanidinium thiocyanate, 10 mM Tris-HCl (pH 8.0) for 10 min.”
“[2] guanidinium chloride (70-80% inhibition at 0.1 M [1]) [1] p-chloromercuribenzoate”
“(stabilizes during preparative manipulations [2]) [2] General stability information, dialysis against 1.5 M guanidinium hydrochloride removes FAD, no re - naturation possible [1] 279”
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