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Intracellular and axonal alpha-synuclein increases in the intermediolateral nucleus and the dorsal horn lamina I layer of the spinal cord after oral rotenone treatment.
(DMV), the intermediolateral nucleus of the spinal cord and the substantia nigra, providing the basis for the neuropathological staging of the disease.
In an attempt to understand if a strictly neural route of CNS invasion might have occurred, we performed sPMCA on the mesenteric, mediastinal, and ileocecocolic lymph nodes, tonsils, vagus nerve, intermediolateral spinal cord segments, and ileum from the above 5 urine/feces exposed deer and from positive and negative control deer.
Obex, vagal nerve, intermediolateral spinal cord segments, sections of ileum, and a number of lymphoid tissues, including tonsil and retropharyngeal, mesenteric, mediastinal, and ileocecocolic lymph nodes, were collected at necropsy and frozen at − 70°C.
They are found in certain areas of the central nervous system (CNS), e.g. the dorsal motor nucleus of the vagus (DMV), the intermediolateral nucleus in the spinal cord (IML), the locus coeruleus (LC) and the olfactory bulb (OB), and of the peripheral nervous system (PNS) e.g., celiac ganglia and ENS, of PD patients
(scale bars 20 µm) A, B, C, Immunostaining against alpha-synuclein and choline acetyl transferase (ChAT) in spinal cord sections showing the intermediolateral nucleus ChAT
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