Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. The halogenated derivative of acetamide, CH2I-CO-NH2, that is used, in peptide mapping, to bind to cysteine

Etymologies

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Examples

  • Samples were subjected to reduction with dithiothreitol, alkylation with iodoacetamide, and in-gel digestion with trypsin or chymotrypsin overnight at pH 8.3, followed by reversed-phase microcapillary/tandem mass spectrometry (LC/MS/MS).

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  • After completion of IEF, IPG strips were incubated for 15 min in equilibration solution I (50 mM Tris pH 6.8, 6 M urea, 30% glycerol, 2% SDS, 2% DTT, trace bromophenol blue) and 15 min in equilibration solution II (solution I with 2. 5% iodoacetamide instead of DTT).

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  • On the other hand, it has been reported that iodoacetamide-containing AChE inhibitors do not bond covalently to

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  • Since iodoacetamide is a commonly used sulfhydryl agent, such a finding would suggest that the active-site Cys residue in

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  • (IC50: 3.4 mM [64]) [64] imidazole reagents < 1, 5, 6 > [41] iodoacetamide

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  • The strip is rehydrated for 8-12 hours with the application of 50 V at room temperature, then prepared for SDS-PAGE by incubation in reducing buffer (50 mM Tris, 6 M urea, 100 mM DTT, 30\% glycerol, and 2\% SDS) for 15 min then in alkylating buffer (50 mM Tris, 6 M urea, 250 mM iodoacetamide, 30\% glycerol, and 2\% SDS) for

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  • (at 1 mM 90% inhibition [10]) [10] iodoacetamide (at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects [6]) [6] Metals, ions Fe2+

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  • (substrate inactivates enzyme after a few turnovers [6]) [6] iodoacetamide

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  • [1] diethyldithiocarbamate [1, 2] iodoacetamide [1] p-hydroxymercuribenzoate

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  • < 1, 3 > (0.5 mM, 21% inhibition [1]; 5 mM, 95% inhibition [3]) [1, 3] iodoacetamide

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