Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A gene segment that codes for a variable portion of the protein immunoglobulin.

Etymologies

mini- +‎ gene (Wiktionary)

Examples

  • Cytotoxicity of GCV on cell cultures was determined using Cell Proliferation Kit I (MTT) (Roche, Mississauga, ON) according to the manufacturer's instructions. pAlb-vTK was digested with BstXI and HindIII to yield a 5134 bp minigene containing the murine Alb p/e with the downstream vTK coding region which was microinjected into fertilized oocytes prior to their transfer into the oviducts of pseudopregnant FVB/N mice using standard procedures

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  • Inheritance of the vTK minigene by SCID/uPA mice did not appear to impact inherent ability of endogenous MH to develop into RN since by 3 months old, vTK+SCID/uPA mouse liver had architectural features that typically mark the hepatocellular disease seen with age-matched SCID/uPA mice

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  • RAD51C minigene splicing constructs carrying either the wild-type or the 145+1G >

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  • RAD51C exon 6 with adjacent intronic sequences. (g) RT-PCR analysis of RNA from HeLa cells transfected with the minigene constructs revealed exclusion of exon 6 in the presence of the 905+5G >

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  • First, the GLR-1 trafficking defect observed in rpm-1 mutants can be rescued by cell-autonomous, postsynaptic (but not presynaptic) expression of an rpm-1 minigene.

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  • Plasmids containing the glr-1 promoter, followed either by an rpm-1 minigene, pmk-3 cDNA, dlk-1 cDNA, snn-1, syntaxin-13 cDNA, or rab-5 cDNA (including mutant versions), were generated using standard techniques.

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  • Transient transfection of minigene constructs in HEK 293 cells was used for genomic and small molecule compound high-throughput screens (HTS) described below, due to superior luciferase signal when compared to stable clones in either HEK 293 or C2C12 cellular backgrounds (data not shown).

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  • In agreement with our results, Stoilov et al. also found that compounds identified via HTS with a minigene reporter construct in HEK 293 cells were capable of specifically enhancing inclusion of exon 10 in endogenous transcripts of The most potent small molecules identified by our DMD exon skipping screen were podophyllotoxin tubulin modulators, and HDAC inhibitors TSA and scriptaid.

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  • A reporter minigene was constructed (pGFP-E6A) containing the coding sequence for GFP whose translation was dependent on faithful splicing for removal of the VEGF exon 6A.

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  • The function of this sequence as an ESS was confirmed in vivo both in the context of the reporter minigene as a plasmid and in the context of a longer minigene with VEGF exon 6A in its native context in an adenoviral gene transfer vector.

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