Definitions
from The Century Dictionary.
- noun A tumor formed of overgrowth of the neuroglia; a glioma.
Etymologies
Sorry, no etymologies found.
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Examples
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H4 neuroglioma cells grown on coverslips in 12 well plate, fixed in 4\% formaldehyde in PBS for 15 minutes and permeabilized in 0. 5\% TritonX-100 for 20 minutes.
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H4 neuroglioma cells were seeded on 12 well plates at the density of 10 5 cells per well, and grown for 12 hours at 37C.
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Human H4 neuroglioma cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10\% fetal bovine serum at 37C in 5\% CO
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H4 neuroglioma cells were transfected with the indicated plasmids (FLAG-FE65 expressing FE65 and / or myc-Tsh3 expressing Teashirt3) and immunoprecipitated (IP) with either mouse IgG (mIgG) or anti-myc antibodies (myc).
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H4 neuroglioma cells expressing FLAG-FE65 and myc-Teashirt3 were subjected to cross-linking in
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For cRNA synthesis, H4 neuroglioma cell lines stably expressing GFP or FLAG-FE65 together with myc-Teashirt3 were harvested and total RNA purified using Qiagen RNA easy kits following the manufacturer's protocol. cRNAs were synthesized and purified using True-Labeling-AMP
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To confirm the FE65-Teashirt3 interaction in mammalian cells, we carried out co-immunoprecipitation of myc-tagged Teashirt3 and FE65 from transiently transfected human neuroglioma (H4) cells (
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To explore association of additional endogenous Teashirt proteins with FE65, we studied endogenous FE65 and Teashirt in H4 neuroglioma cells (
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