“For the experiments including piceatannol, the cells were incubated in HBSS containing 50 µM piceatannol for 30 min before control movies were acquired, and piceatannol was present throughout the experiment.”
“Control experiments indicate that the formation of AP-2 containing structures was not affected by treatment with piceatannol or carrier (DMSO) only.”
“There was no statistically significant increase (p = 0.2) in the number of AP-2 spots when adding StxB upon piceatannol treatment.”
“Presence of the Syk inhibitor piceatannol prevented the formation of new clathrin-coated structures induced by StxB, indicating a role for Syk in the formation of clathrin-coated pits.”
“After 30 min of incubation with piceatannol the amount of AP-2 spots relative to the control (100%) was 104±3% (mean ± average deviation, 4 cells analyzed per experiment; two experiments).”
“The cells were then exposed to StxB for 10 min, followed by the acquisition of a second time-lapse series of 1-2 min. Six independent experiments, with 1-4 cells per experiment, were analyzed, and the result is expressed relative to piceatannol only.”
“Syk inhibitor piceatannol (50 µM) for 30 min followed by the acquisition of a spinning disk confocal time-series of 1-2 min duration.”
“HeLa cells stably expressing σ2-EGFP were pre-incubated with piceatannol (50 µM) for 30 min.”
“Inhibition of Syk by piceatannol inhibits the Stx-induced increase in the number of AP-2 spots.”
“The content of resveratrol including that of its derivatives, resveratrolosid, piceatannol, piceid and astringin, was significantly higher in the presence of melilot on clay, loess and clayCS.”
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