“Among others, many prokaryotic messages are polycistronic, few eukaryotic.”
“The strategy of these authors entailed the introduction of a polycistronic vector encoding the genes c-Myc, Klf4, and SV40LT and selection of stable episomes that provided persistent gene expression to achieve reprogramming followed by the identification of vector-free subclones after several passages in the absence of selection.”
“Notably, these studies demonstrated that somatic cells carrying a single proviral copy of the polycistronic cassette can be reprogrammed to pluripotency, substantially reducing genomic modification of the target cell.”
“A feasible way to decrease the risk of insertional mutagenesis is to combine the reprogramming factors into a single polycistronic vector by inserting a 'self-cleaving' 2A peptide or an internal ribosome entry site (IRES) sequence between two consecutive open reading frames.”
“Stem Cell Cassette (STEMCCA) polycistronic lentiviral vector”
“Similarly, a Cre-expressing plasmid or adenovirus was employed to remove three or four copies of a polycistronic vector from the genome of mouse iPSCs”
“A number of studies have recently demonstrated the advantage of using polycistronic vectors for iPSC generation.”
“We find mir-424 and mir-503 are derived from a polycistronic precursor mir-424-503 that is under repression by the MLL-MLLT3 leukemogenic fusion.”
“Alternative initiation strategies by ribosomal frameshifting and leaky scanning enable polycistronic translation of the cap-dependent viral transcript.”
“This mechanism paves the way for using polycistronic MIRNA precursors as a new molecular tool for plant biologists to simultaneously control the expression of different genes.”
‘polycistronic’ hasn't been added to any lists yet.
Looking for tweets for polycistronic.