subclones

DBT subclones stably expressing cannabinoid receptors were expanded in 24-well plates, incubated with CP-55,940 (CP, 1 µM), and ERK1/2 phosphorylation quantified by Western blot analysis.

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

Wild-type (wt) DBT cells and DBT subclones stably expressing cannabinoid receptors were expanded in 24-well plates, incubated with CP-55,940 (CP) at either 1 µM (receptor mediated) or 10 µM (receptor independent), and cell viability was assessed by quantifying WST-1 conversion.

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

Remarkably, cannabinoids applied at high concentration induce apoptosis in all subclones independently of CB

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

DBT subclones stably expressing cannabinoid receptors were expanded in 24-well plates, incubated with CP-55,940 (CP, 1 µM), and AKT phosphorylation quantified by Luminex multiplex immunoassay.

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

Thus, we generated DBT subclones that stably express set levels of either CB Expression levels of CB1 and CB2 receptors in wild-type and selected DBT subclones.

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

To do so, we treated all DBT subclones with CP-55,940 and assessed the phosphorylation state of these kinases using a Luminex multiplexed immunoassay system.

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

Genomic DNA of these clones and their subclones (1-3) with parental NCR1 cells were analyzed.

PLoS ONE Alerts: New Articles Munehiro Uemura et al. 2010

The strategy of these authors entailed the introduction of a polycistronic vector encoding the genes c-Myc, Klf4, and SV40LT and selection of stable episomes that provided persistent gene expression to achieve reprogramming followed by the identification of vector-free subclones after several passages in the absence of selection.

BioMed Central - Latest articles Cesar Sommer 2010

Taxol-resistant subclones, derived from the cancer cell line MDA-MB-435, sustained continuous growth in high concentrations of Taxol while the Taxol-sensitive cells could not.

BioMed Central - Latest articles Ming Zhou 2010

Conversely, local injection of high concentrations of cannabinoids will induce apoptosis in all astrocytoma subclones

PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010