Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A reduction in the mobility of a protein-DNA complex relative to unbound DNA, due to the binding of an antibody to that complex
  • v. To induce a supershift

Etymologies

super- +‎ shift (Wiktionary)

Examples

  • EMSA with Sp1 binding motif using U937 cells and supershift with Sp1, Sp3 and Sp4 antibodies.

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  • For supershift experiments, nuclear extracts were incubated with an anti-p65 antibody.

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  • Furthermore, a supershift was induced by an anti-p65 antibody, confirming that 5-FU induced NF-κB complexes contained the 65-kDa subunit (p65).

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  • We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.

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  • Standard EMSAs and supershift EMSAs with relevant antibodies were performed using the LightShift Chemiluminescent EMSA Kit (Pierce, Rockford, IL).

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  • The ERα-containing complex (arrow) is demonstrated by supershift (arrowhead) using a monoclonal antibody that recognizes ERα.

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  • For supershift experiments, an anti-p65 antibody was added (Santa Cruz

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  • Even in the reaction in which more αCTD bound to the mutant promoter than the wild-type promoter, Spx was unable to supershift the mutant promoter complex as efficiently as the wild-type promoter complex (

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  • No supershift was noted using antibodies targeting the transcription factors Sp1, AP-1 or ARP-1 / COUP-TF2, although these factors have been demonstrated to bind with ERα at selected promoters (

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