Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biochemistry, proteomics A reduction in the mobility of a
protein -DNA complex relative to unbound DNA, due to the binding of anantibody to that complex - verb biochemistry, proteomics To induce a supershift
Etymologies
from Wiktionary, Creative Commons Attribution/Share-Alike License
Support
Help support Wordnik (and make this page ad-free) by adopting the word supershift.
Examples
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
BioMed Central - Latest articles Rainer Furbass 2010
-
For supershift experiments, nuclear extracts were incubated with an anti-p65 antibody.
PLoS ONE Alerts: New Articles Nadine Rohwer et al. 2010
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
-
EMSA with Sp1 binding motif using U937 cells and supershift with Sp1, Sp3 and Sp4 antibodies.
PLoS ONE Alerts: New Articles Anil K. Singh et al. 2010
-
Furthermore, a supershift was induced by an anti-p65 antibody, confirming that 5-FU induced NF-κB complexes contained the 65-kDa subunit (p65).
PLoS ONE Alerts: New Articles Nadine Rohwer et al. 2010
-
We enriched the E-BP from placental nuclear extracts using DNA-affinity Dynabeads and showed by Western blot analysis and supershift EMSA experiments that the E-BP is composed of the transcription factors upstream stimulating factor (USF) 1 and USF2.
-
Standard EMSAs and supershift EMSAs with relevant antibodies were performed using the LightShift Chemiluminescent EMSA Kit (Pierce, Rockford, IL).
Comments
Log in or sign up to get involved in the conversation. It's quick and easy.