- Greek thūlakos, sack + -oid. (American Heritage® Dictionary of the English Language, Fourth Edition)
“Light energy from the sun is captured by photosynthetic pigments and is transferred along a series of proteins and iron-sulfur containing compounds along the thylakoid membranes of the chloroplast; the net result being the formation of high-energy compounds such as ATP and NADPH.”
“Under these conditions, high light conditions can cause thylakoid membranes to become damaged in a process known as photoinhibition.”
“In the exposed peduncle, the membranes constituting the thylakoids were very distinct and plentiful, but in the flag leaf, there was a sharp breakdown at stage 4 and complete disintegration of the thylakoid membranes at stage 5.”
“The thylakoid membranes were adjusted to a Chl concentration of 15 µg ml”
“After washing with 2 mM dodecyl maltoside to remove any of the remaining phycobilisomes, the membranes were washed twice and resuspended in the thylakoid buffer to a Chl concentration of 1 mg ml”
“The thylakoid protein complexes were then separated by step sucrose gradient ultracentrifugation at 160,000×”
“To fractionate the membrane protein complexes, a solution of 10% (the percentage (%) of a solution is calculated as weight to volume (w/v) unless otherwise indicated) dodecyl maltoside was added to the thylakoid membranes to achieve a detergent-to-Chl ratio of 15: 1.”
“Blots of the thylakoid membrane proteins were probed with polyclonal anti-D1 antibodies.”
“The cells were broken in a Bead-Beater with an ice-jacketed sample chamber by six breakage cycles at full speed (30 s of bursts, followed by 5 min of chilling) after adding an equal volume of glass beads pre-wetted by the thylakoid buffer to the cell suspension.”
“The thylakoid protein complexes were then isolated, solubilized in mild detergents and fractionated by sucrose gradient ultracentrifugation.”
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