from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. a cell (bacteria, fungus, yeast or plant) into which foreign DNA has been introduced
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The recombinant plasmid was transformed into E. coli BL21 (DE3) pLysS cells and the transformant was stored at − 80°C.
Individual transformant clones were grown overnight at 37°C in 10 ml of Luria-Bertani (LB) medium containing 50 µg ml
The complementary polypeptide whose amino acid sequence is thus determined may be obtained by diverse means such as, for example, chemical synthesis, derivation from a protein or larger polypeptide containing said amino acid sequence, or, when the second nucleic acid is DNA, inserting the second nucleotide sequence into a plasmid to form a recombinant DNA plasmid vector and transforming a unicellular organism therewith to produce a transformant unicellular organism biosynthesizing said complementary polypeptide.
EphA4-HA transformant cells were transfected with pBOS-meltrin β
(A) EphA4-HA transformant cells were transfected with Meltrin β expression plasmids (meltrin β) or with control vectors, and biotinylated proteins on the cell surface were precipitated with streptavidin beads.
Un grand grand merci à Thierry qui a gentiment accepté de fournir le matériel, de filmer, et même, devant mon angoisse de l’objectif noir, d’improviser quelques questions, transformant la petite séquence en interview.
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