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- n. The organosilicon radical (CH3)3Si-
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The first step (1) was an Arbusov reaction between an activated carboxylic function and a very reactive species, the bis (trimethylsilyl) phosphite and the second one (2) was hydrolysis. doi: 10.1371 / journal. pone.0004685.g001
Briefly, a very reactive species, the bis (trimethylsilyl) (p-methoxyphényl) phosphite, was reacted following an Arbusov (1) reaction with an activated carboxylic function and then the intermediate was hydrolysed (2).
The trimethylsilyl-derived sterols were separated on an HP-5MS 5%-phenyl methyl polysiloxane capillary column (30 m×0. 25 mm inner diameter x 0.25 µm film) with carrier gas helium at the rate of 1 ml/min.
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