from The American Heritage® Dictionary of the English Language, 4th Edition
- n. A microtome for cutting very thin sections of material for use in electron microscopy.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. A microtome that cuts extremely thin slices of tissue, especially for use in electron microscopy.
For transmission electron microscopy, a diamond knife mounted in an ultramicrotome is used to cut 50-nm-thick tissue sections which are mounted on a 3-mm-diameter copper grid.
The specimen was serially sectioned with a diamond knife (Diatome, HistoJumbo) at 1 µm intervals on a Reichert-Jung ultramicrotome.
The ultramicrotome was then used to prepare thin sections, which were collected on a copper grid and stained with lead citrate and uranyl acetate Comparisons between variables were tested by Student t test of means, the paired Student t test or Mann-Whitney U test, as appropriate.
After dehydratation in a growing gradient of ethanol, 5 min for each step: 30, 50, 70 and 95\%, impregnation steps and inclusion were performed in Epon finally polymerized at 60°C for 48 h. 60-80 nm sections were obtained using an ultramicrotome RMC-MTX (Tucson), and contrasted with uranyl acetate and lead citrate.
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