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  • "We developed techniques to analyze the structure at high resolution using cryo-EM," explains Yonekura.

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  • Cryo-electron microscopy (cryo-EM) can be used, but the resolution is usually not high enough to see atomic details, because electron irradiation severely damages biological samples.

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  • PDBe is the founder of the worldwide archive of 3D cryo-EM data, the Electron Microscopy

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  • Using a combination of crystallography and cryo-EM, the authors concluded that kinesin-8 motors respond to both MT and tubulin substrates, reflecting their multi-tasking activities.

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  • The cryo-EM adenovirus structure is one of only a handful of atomic resolution cryo-EM structures that have been solved to date.

    Slashdot: Science

  • Experience in at least two of the following techniques is essential: crystallization of membrane proteins in 2D, electron cryo-crystallography, and cryo-EM image analysis.

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  • Tasks: Membrane protein expression, purification, cryo-EM imaging, 2D crystallization, cryo-electron crystallography data collection, image processing, 3D structure reconstruction, model building, high-resolution interpretation of the functional consequences of the maps.

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  • "Our goal is to set up a structural biology program that includes macromolecular NMR, macromolecular X-ray crystallography, and cryo-EM."

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  • Growing the cells directly on EM grids has been very successful, especially for cryo-EM

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  • The ribosome, a molecular machine that synthesizes proteins in the cell, provides many examples of such dynamic assemblages, and the complex between the bacterial ribosome and translation elongation factor Tu (EF-Tu), stabilized by the antibiotic kirromycin, was the first to be visualized by cryo-EM (1).

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  • cryo-electron microscopy

    October 7, 2017