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- n. Plural form of thymus.
Sorry, no etymologies found.
Adult thymi were cut into small pieces and majority of thymocytes were released by gentle agitation using a glass pasture pipette.
A total of 5 thymi from each strain were pooled for each experiment to yield sufficient numbers of TECs for analysis and 3 independent experiments were performed.
Embryonic thymi were digested with collagenase D (1.2 mg/ml, Roche Diagnostics), DNase I (1.5 mg/ml, Roche Diagnostics), and Dispase (1.25 mg/ml, Invitrogen) at 37°C for 15 minutes with occasional gentle agitation with a glass pasture pipette.
After 72 hrs, thymi were washed in 10% complete RPMI and lobes were embedded in OCT and snap frozen for sectioning.
Means represent the total cell number/thymus, calculated from 3 independent experiments utilizing 5-pooled dissociated thymi from each strain.
The authors additionally examined clonal deletion in artificial re-aggregated thymi.
The negative controls used were spleens and thymi from non-GFPtg-nursed B6 mice, and the positive controls used were spleens and thymi from GFPtg mice. *, p FACS analysis interestingly and clearly showed that CD4+ cells preferentially migrated to the spleen (