imidazole

• For the purification of His-tagged HDAC1, cells were resuspended in lysis buffer containing 50mM sodium phosphate (pH8. 0) / 300mM NaCl / 10mM imidazole and lysed with three rounds in the French press. —  PLoS ONE Alerts: New Articles
• Their imidazole sidechains experience simple, uncoupled Henderson-Hasselbalch type protonations, giving rise to four different protonation states. —  CiteULike: Everyone's library
• This behavior can be explained by a conformational change upon protonation that moves the imidazole sidechain of H64 away from the CO into the high-dielectric solvent environment, which avoids the energetically unfavorable situation of an uncompensated electric charge in the apolar, low-dielectric protein interior. —  CiteULike: Everyone's library
• The Leu337 residue is conserved among plant and bacterial KAS proteins, and in the crystal structures of E. coli KAS I and KAS II, this leucine abuts a phenylalanine whose imidazole ring extends into the substrate binding cavity causing the fatty acid chain to bend. —  CiteULike: Everyone's library
• After several washes with buffer containing 8 M urea and 10-60 mM imidazole, bound protein was eluted sequentially with 500 mM and 1 M imidazole in the presence of urea. —  PLoS ONE Alerts: New Articles

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