Definitions

from The American Heritage® Dictionary of the English Language, 4th Edition

  • n. A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies.

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A blot used in western blot analysis
  • v. To perform such an analysis

Etymologies

immuno- +‎ blot (Wiktionary)

Examples

  • GM immunoblot showed an IgE-binding 14 kDa band that was totally inhibited after serum pre-incubation with GM.

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  • Screen with FAST-ELISA and confirm with immunoblot.

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  • A recombinant immunoblot assay (RIBA) that identifies antibodies which react with individual HCV antigens is often used as a supplemental test for confirmation of a positive EIA result.

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  • Figure 6: Magoh acts upstream of the microcephaly-associated protein Lis1 to regulate neurogenesis. gene expression measured by quantitative PCR (left) and Lis1 protein expression measured from immunoblot analyses (right).

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  • Substitutions affecting respiration are in bold. (a) Fluorescence immunoblot analysis with anti-MrpL32 shows that yeast MrpL32 precursor (p) accumulates in yta10Δyta12Δ m-AAA-deficient cells (lane 2). m, mature MrpL32.

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  • P > 0.05. (b) Fluorescence immunoblot analysis and protein quantitation of mitochondria-encoded COX subunits Cox1p, Cox2p and Cox3p, and nuclear-encoded subunit Cox4p, in mitochondrial extracts from cells expressing AFG3L2 alone, in the absence of paraplegin.

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  • Lanes 1-5, immunoblot analysis of cell extracts before immunoprecipitation (input).

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  • Huh7 cells were transfected with pCI-vTK and G418-resistant Huh cellular clones (cl) (1-6) were evaluated for vTK expression (~43 KDa, arrowheads) by immunoblot analysis.

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  • Cellular expression of vTK protein was detected by immunoblot analysis essentially as described

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  • Different band intensities corresponding to immunoblot detection of protein samples were quantified using the MCID software (MCID, Cambridge, UK).

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