Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A univalent cation derived from a tetrazole

Etymologies

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Examples

  • You can’t tell the difference until you take a sample of the bacteria and ‘plate them out’ on an agar plate that contains a nutritious broth plus the sugar arabinose and a chemical dye called tetrazolium.

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  • The retinas were postfixed for 30 minutes in the same fixative, washed and incubated in a solution containing 0. 02% NADPH-diaphorase and 0. 04% nitroblue tetrazolium

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  • Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence.

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  • After incubation for 72 hrs, the tetrazolium-based cell viability (MTT) assay was performed.

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  • The sections were developed at 37°C for ~4 h in the same buffer with nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) diluted 1 / 50 from the stock solution (Roche Diagnostic).

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  • MDA-MB-231 and D3H2LN cell viability was assessed using the MTT-microculture tetrazolium assay

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  • The assay utilizes a novel tetrazolium compound that metabolically active cells convert to a water-soluble formazan by the action of cellular dehydrogenases, which is measured by absorbance at 490 nm using a colorimetric microtiter plate reader

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  • After 5 days of co-culture T cell proliferation was analyzed by performing MTT (3 - [4,5-dimethylthiazol-2-yl] - 2,5-diphenyl tetrazolium bromide) assay (Roche Molecular Biochemicals).

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  • Following washes, 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium alkaline phosphatase substrate solution (Sigma) was added and samples read by a VERSA max microplate reader (Molecular Devices, Sunnydale, CA) at OD

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  • Panels B-D: Cell viability values at 72 hours of incubation, as determined by the methyl tetrazolium (MTT) method: ACH-2 cells (B), Jurkat 6.3 cells (C), uninfected Jurkat cells (D).

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