Definitions
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Etymologies
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Examples
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Creatividad en experimentación... muy bueno uM. 1 year ago
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Say 1 mM Ca2+ gives pain, 100 uM a subtle tactile signal.
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Eight to ten hours later, 2 mM thymidine was added again for 14-16 hours, cells were washed twice with PBS, then released into thymidine-free media. 50 uM roscovitine was administered for 6 hours following 16 hours nocodazole treatment in A549 cells.
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Blood was obtained via retro-orbital bleeding of anesthetized mice, or spleens and lymph nodes were removed from euthanized animals, homogenized, and passed through a 40 uM filter to create
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Immunoprecipitates were then incubated with 20 uL kinase reaction mix (kinase buffer, 10 uM ATP) with or without 175 ng recombinant Cdc2/cyclin B (Cell Signaling Technologies #7518) at
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After washing the beads twice in lysis buffer 1, they were washed twice in CIAP buffer (50 mM Tris pH 8.5, 100 uM EDTA) then incubated with 5 uL calf-intestinal alkaline phosphatase (CIAP) (NEB) with constant agitation at 37°C for 30 minutes.
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Combined treatment with subeffective doses of erlotinib (0.25 uM) or gefitinib (0.5 uM) with subeffective doses of gamma-tocotrienol
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A solution of Thioflavin T (Sigma) was prepared at 400 uM in water
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Treatment with 3.5 uM gamma-tocotrienol, 0.5 uM erlotinib or 1.0 uM gefitinib alone, significantly inhibited +SA tumor cell growth.
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The fact that O2 dissolves in water, about 250 uM at 15 degrees, also buggers up measurement as the level of water vapor is variable.
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