“The male macrogamont fertilizes the female one, and they produce a round little offspring called an ookinete.”
“An ex vivo ookinete was obtained from an Anopheles stephensi infected bloodmeal 32 hours post-feeding, resuspended in Matrigel (BD Bioscience, UK) and imaged for 10 minutes.”
“Prior studies show that the ookinete-to-oocyst phase in the malaria life cycle, when the malarial parasite is fertilized in the mosquito's body, is one of the most vulnerable stages making it an ideal target for antitransmission vaccines.”
“In the study researchers developed a nasal vaccine based on ookinete-surface proteins (OSPs or also known as parasite antigens) and intranasally vaccinated mice infected with malaria.”
“Malaria ookinete surface protein-based vaccination via the intranasal route completely blocks parasite transmission in both passive and active vaccination regimens in a rodent model of malaria infection.”
“The experimental vaccine, which contained parasite antigens, blocked the ookinete-to-oocyst phase in the malarial life cycle in which the malarial parasite is fertilized in the mosquito's body, the scientists wrote in the December issue of the journal Infection and Immunity.”
“The ookinete divides in the mosquito’s gut into thousands of sporozoites, which travel to the mosquito’s salivary glands, there to be injected into some new human host.”
“(A) liver stage trophozoites in a primary human hepatocyte (B) blood ring stage, (C) trophozoites, (D) schizont, (E) male gametocyte, (F) female gametocyte, (G) ookinete obtained 29 hours post-feed in infected”
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