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To amplify a long nDNA fragment, the thermocycler profile included initial denaturation at 94°C for 1 min, 27 cycles for β-globin or 26 cycles for β-polymerase of 94°C 15 sec, 65°C 12 min, with final extension at 72°C for 10 min.— PLoS ONE Alerts: New Articles
To amplify a short nDNA fragment (84 bp), the same program as β-globin was used except that extension temperature was— PLoS ONE Alerts: New Articles
Sickle cell disease, which affects millions of people worldwide, arises because of a tiny genetic defect in the gene for beta-globin, a protein component of hemoglobin.
This immature hemoglobin, which usually disappears after birth, does not contain beta-globin, but another form called gamma-globin.
He and his colleagues developed a technique to insert the gene for gamma-globin into blood-forming cells using a harmless viral carrier.

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