from The American Heritage® Dictionary of the English Language, 4th Edition
- n. Any of a series of open chain hydrocarbons with a carbon-carbon triple bond and the general formula CnH2n-2.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. A hydrocarbon containing at least one carbon–carbon triple bond.
from WordNet 3.0 Copyright 2006 by Princeton University. All rights reserved.
- n. a colorless flammable gas used chiefly in welding and in organic synthesis
Follow the leader: the use of leader peptides to guide natural product biosynthesis Rapid behavior-based identification of neuroactive small molecules in the zebrafish Capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry Domino access to highly substituted chromans and isochromans from carbohydrates
Since its introduction in 2001 by the Nobel laureate chemist Barry Sharpless of the Scripps Research Institute, the copper-catalyzed azide-alkyne reaction has proven extremely valuable for attaching small molecular probes to various biomolecules in a test tube or on fixed cells.
Click chemistry is best known for a copper-catalyzed azide-alkyne reaction that makes it possible for certain chemical building blocks to "click" together in an irreversible linkage, analagous to the snapping together of Lego blocks.
Slide 3: Unsaturated Hydrocarbons Unsaturated hydrocarbon: contains one or more x carbon-carbon double or triple bonds Alkene: contains a carbon-carbon double bond x and has the general formula CnH2n H H C C H H Ethylene (an alkene) Alkyne: HC CH a carbon-carbon triple bond and contains x has the general formula CnH2n-2 Acetylene (an alkyne)
That first peptide - which is about a third of the length of the final capture agent the scientists are trying to make - is then isolated, purified, and modified on one end by the addition of a chemical group called an alkyne.
The alkyne group on the added peptide can potentially chemically react with the azide group of the library's peptides, to create a new peptide that is now two segments long.
The two-segment-long peptide then is isolated and purified, "and then we modify the end of that with an alkyne and add it back to the library to produce a three-segment peptide, which is long enough to be both selective for and specific to the target protein," Dr. Heath said.
The supernatants were then treated with the alkyne - or azide-conjugated biotin-tags, and click chemistry was used to label the targets of the 4-BPB click derivatives.
The propargyl (alkyne) and azido groups are bioorthogonal reaction partners.
List of top 100 targets identified by LC-MS/MS using 4-PPB, the alkyne click derivative of
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